NASC Stock Detail Page
NASC ID: N799176
Name: pH7-p35S-mDII-VENUS-N7
ABRC stock number: CS799176
Description: Vector expressing a fusion of a mutated auxin-resistant IAA28 domain II to a nuclear targeted VENUS under the 35S promoter. This was used to produce the mDII-VENUS line, NASC catalogue number N799174 (see reference for more details)
Donation Date: 2012-01-25
| Donated by: | Ecole Normale Supérieure de Lyon Teva Vernoux Ecole Normale Supérieure de Lyon Geraldine Brunoad |
Donor Number: pH7-p35S-mDII-VENUS-N7
Part of : DII-VENUS
Stock type: individual line
Material type: plasmid dna
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Marker used: Spectinomycin
Growth requirement: Host Strain: E.coli DH5a. Growth Temperature: 37°C. Duration of Growth: Over night (16h). Media: LB +Spectinomycin 100mg/l
References
Brunoud, G., et al. 2012. A novel sensor to map auxin response and distribution at high spatio-temporal resolution. Nature 482(7383):103-6.PubMed ID: 22246322.